Movie 4

Macrophages interacting with vessels growing in a B16-F1 xenograft.
Confocal time-lapse imaging of a B16-F1 xenograft implanted in an mpeg1:NTR:mCherry;kdrl:EGFP embryo from 24 hpi to 46.33 hpi. Time-lapse image z-stacks were collected 10 min apart; movie was made at 7 frames per second. The vessel that is most ventral at 27.5 hpi was analysed in Fig. 4 between 27.5 hpi to 32.5 hpi. Scale bar = 50 um.