Movie 1

Uninjured Tg(cdh17:mCherry);Tg(lyz:EGFP) transgenic larvae were used to analyze neutrophil interaction (green) with PT (red) from 2.5-3.5 dpf. Neutrophils are observed circulating near the tubules, but without any prolonged contact with the PT. Leica TCS SP5 multiphoton microscope and HCXC IRAPO L 20X/0.95 water objective were used to capture images every 27 minutes for 17 hrs.