Movie 2

Gent-AKI Tg(cdh17:mCherry);Tg(lyz:EGFP) transgenic larvae were used to analyze neutrophil interaction (green) with PT (red) from 1-2 dpi. Higher number of neutrophils are observed near the tubules, with increased contact with the PT. Leica TCS SP5 multiphoton microscope and HCXC IRAPO L 20X/0.95 water objective were used to capture images every 27 minutes for 17 hrs.