Movie 3

Uninjured Tg(cdh17:mCherry);Tg(mpeg1:dendra2) transgenic larvae were used to analyze macrophage interaction (green) with PT (red) from 4-5 dpf. Macrophages are observed circulating near the tubules, but without any prolonged contact with the PT. Nikon Eclipse Ti multiphoton microscope and 20X dry objective was used to capture images every 90 minutes for 24 hrs.