Movie 4

Gent-AKI Tg(cdh17:mCherry);Tg(mpeg1:dendra2) transgenic larvae were used to analyze macrophage interaction (green) with PT (red) in gent-AKI setting from 2-3 dpi. More macrophages are observed adjacent to the PT, with prolonged contact time. Nikon Eclipse Ti multiphoton microscope and 20X dry objective was used to capture images every 90 minutes for 24 hrs.