Movie 1

Example of a 3D reconstruction of an embryonic heart at E9.5 by HREM. The first sequence shows a 3D projection, rotating rightward, of an E9.5 embryo imaged by HREM. The second sequence shows serial transverse sections of the thoracic region. The final sequence displays a 3D reconstruction of the embryonic heart shape, after image segmentation. It is first presented within the image of the thoracic region, and then isolated, using the notochord as an axial reference. AVC, atrioventricular canal. x/y resolution,1.45 μm; z resolution,1.56 μm.

Standardised imaging pipeline for phenotyping mouse laterality defects and associated heart malformations, at multiple scales and multiple stages

Audrey Desgrange, Johanna Lokmer, Carmen Marchiol, Lucile Houyel, and Sigolène M. Meilhac

Disease Models and Mechanisms 2019. 12:None-None; doi: 10.1242/dmm.038356