Movie 5

Application of the multimodality imaging pipeline in a Rpgrip1l-/- embryo. The first sequence is a fast scan of the Rpgrip1l-/- E9.5 embryo shown in figure 6B, from ventral to dorsal. x/y resolution, 50 μm. The second sequence is a micro-CT scan of the same individual at E18.5, showing in parallel coronal (on the left) and transverse (on the right) optical sections. x/y/z resolution, 20 μm. The left isomerism of the lungs, hypoplasia of the pulmonary artery branches (PAB), the azygos return of the right superior caval vein are indicated. The final sequence shows serial transverse sections of the isolated heart at E18.5 acquired by HREM and the corresponding 3D projection. x/y resolution, 2.9 μm; z resolution, 2.34 μm. The atrioventricular septal defect is indicated with an asterisk. Ao, aorta; Co, colon; LA, left atrium; LLuL, left lung lobe; LV, left ventricle; PA, pulmonary artery; RA, right atrium; RV, right ventricle; St, stomach, Sp, spleen.

Standardised imaging pipeline for phenotyping mouse laterality defects and associated heart malformations, at multiple scales and multiple stages

Audrey Desgrange, Johanna Lokmer, Carmen Marchiol, Lucile Houyel, and Sigolène M. Meilhac

Disease Models and Mechanisms 2019. 12:None-None; doi: 10.1242/dmm.038356