Surviving Ccdc151 knockout animals do not contain any sperm in epididymis and vas deferens. Sperm was collected from 6 weeks old males and deposited on a petri dish in PBS media and filmed at 20X magnification on an inverted microscope. Movie 4: No sperm was observed in preparation from the Ccdc151 homozygous knockout male; Movie 5: Sperm collected from the WT littermate.
Francesco Chiani, Tiziana Orsini, Alessia Gambadoro, Miriam Pasquini, Sabrina Putti, Maurizio Cirilli, Olga Ermakova, and Glauco P. Tocchini-Valentini
Disease Models and Mechanisms
2019. 12:None-None; doi: 10.1242/dmm.038489