3D CLEM of Toxoplasma gondii replication in the zebrafish hindbrain, related to Figure 2A. SBF SEM of tachyzoites in the HBV of larvae injected with type I Toxoplasma-GFP at 6 hpi. Representative examples of single (left), replicating (middle) and replicated doublet (right) Toxoplasma from a total of 36 found in zebrafish cells and imaged in their full volume to accurately determine their stage (see Fig. 2A and Fig. S2). 112 consecutive 50 nm SBF SEM slices of a different subregion of a section for each example. Scale bar, 1 μm.