Toxoplasma tachyzoites reside within neurons, related to Figure 2B. In vivo confocal microscopy imaging of live 3 dpf Tg(elavl3:GCaMP6s)jf4 larvae (neurons marked in green) infected in the HBV with type I Toxoplasma-Tomato (red) at 4 hpi. A Z-stack of 33 Z-slices (Z=0.43 μm) is shown at 6 fps showing brightfield (grey) and Toxoplasma (red) composite (left) or Toxoplasma (red) and neurons (green) composite (right). Ventricular surface labeled as VS. The five tachyzoites shown in Fig. 2B are labeled with white arrowheads. Scale bar, 10 μm.