Movie 4

3D CLEM of replicative Toxoplasma gondii inside a zebrafish macrophage, related to Figure S5B. SBF SEM of intraphagocytic parasite replication in the HBV of mpeg1:G/U:mCherry larvae infected with type I Toxoplasma-GFP at 6 hpi. 373 consecutive 50 nm SBF SEM slices in which the replicating Toxoplasma (green and light green) and plasma membrane of the macrophage (red) were manually segmented. A surface was generated to build a 3D model of the 2 segmented Toxoplasma in Amira software. Scale bar, as indicated.

The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages

Nagisa Yoshida, Marie-Charlotte Domart, Christopher J. Peddie, Artur Yakimovich, Maria J. Mazon-Moya, Thomas A. Hawkins, Lucy Collinson, Jason Mercer, Eva-Maria Frickel, and Serge Mostowy

Disease Models and Mechanisms 2020. 13:None-None; doi: 10.1242/dmm.043091