Movie 6

Host cell-intrinsic response disrupts Toxoplasma parasitophorous vacuoles in zebrafish brain cells in vivo, related to Figure 6. ssTEM of parasite replication in brain cells in the HBV of mpeg1:G/U:mCherry larvae infected with type I Toxoplasma-GFP at 6 hpi. Movie shows 71 consecutive 70 nm sections imaged by ssTEM at 6800x magnification at 3 fps. Toxoplasma tachyzoites were imaged in their full volume to accurately assess the continuity of the PV. Color boxes (left- cyan box, middle- magenta box, right- green box) match the ones in Fig. 6. Scale bar, 1 μm.

The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages

Nagisa Yoshida, Marie-Charlotte Domart, Christopher J. Peddie, Artur Yakimovich, Maria J. Mazon-Moya, Thomas A. Hawkins, Lucy Collinson, Jason Mercer, Eva-Maria Frickel, and Serge Mostowy

Disease Models and Mechanisms 2020. 13:None-None; doi: 10.1242/dmm.043091