Movie 6

Host cell-intrinsic response disrupts Toxoplasma parasitophorous vacuoles in zebrafish brain cells in vivo, related to Figure 6. ssTEM of parasite replication in brain cells in the HBV of mpeg1:G/U:mCherry larvae infected with type I Toxoplasma-GFP at 6 hpi. Movie shows 71 consecutive 70 nm sections imaged by ssTEM at 6800x magnification at 3 fps. Toxoplasma tachyzoites were imaged in their full volume to accurately assess the continuity of the PV. Color boxes (left- cyan box, middle- magenta box, right- green box) match the ones in Fig. 6. Scale bar, 1 μm.