Movie 1

Time-lapse imaging of endosomal tubule activity in prebiogenesis salivary glands. Live-cell time-lapse imaging of LAMP-GFP in salivary glands prior to the onset of glue biogenesis shows little tubule activity. Movie shows a maximum intensity projection of three optical slices; movie plays at a frame rate of 30 frames/sec. Scale bar 5 μm. A time-intensity projection of this movie is displayed in Fig. 1C.