Movie 2.

The design and 3D-printing the drug-pressing mould. The 3D-modelling and design of the drug-pressing mould was carried out on the open-source website-based software Tinkercad to generate an STL file, which contains the three-dimensional surface geometry information of the designed object (Dataset 1). The STL file was then imported into a slicing modelling software to choose the customised parameters for paired 3D-printers. In our case, we used the software Ultimaker Cura for slicing and generated a G-code file ready for the 3D-printer Ultimaker 3 to print out the object, with the nozzle set-up as AA 0.25 and to use the generic PLA as the printing material. The printing process itself is highly automated and only requires the user to select the file to print. After the printing, the PLA material must cool and become firm before retrieving the object. Occasional disfigurations might occur during the process of printing which is largely due to residual grease fingerprint left on the bottom of the printing surface due to human touch (using 70% ethanol wiping clean the surface will help reduce such incidents).

Long-term non-invasive drug treatments in adult zebrafish that lead to melanoma drug resistance

Yuting Lu, and E. Elizabeth Patton

Disease Models and Mechanisms 2022. 15:None-None; doi: 10.1242/dmm.049401