Movie 1.
The filopodia-spine transition revealed by live imaging of dendrite protrusion dynamics. Timelapse recordings of the same EGFP-caax labeled PyrN apical dendrite imaged at 4, 6, and 8 dpf. Image volumes were acquired every 10 min for 4 hrs. Note prevalence of long, motile filopodia at 4 dpf, whereas short spines predominate at 8 dpf. At 6 dpf the dendrite contains a mix of long and short protrusions.
A genetic labeling system to study dendritic spine development in zebrafish models of neurodevelopmental disorders
Disease Models and Mechanisms 2022. 15:None-None; doi: 10.1242/dmm.049507