Movie 5.

Representative recordings of cellular migration in B2 line. Migration tracks of individual cells were recorded by marking the position of the nucleus in individual frames. Colored lines indicate positions of individual cells during the duration of migration assay. From the migration pattern in B2, we encountered mixed patterns of cellular migration: apart from linear trajectory, these NPCs also demonstrated back-and-forth as well as circular motions. Images for migration of B2 was captured with 10x objective. While the migration pattern in C1-C3 is largely linear (S1-A, B, C), showing individual cells from either side of 500µm gap; in B1 and B2 lines (S1-D and S1-E), we encountered mixed patterns of cellular migration: apart from linear trajectory, these NPCs also demonstrated back-and-forth as well as circular motions. Images for migration of C1 and B1 cells were captured with 4x objective and C2, C3, B2 cell migration captured with 10x objective.