Movie 2.

Imaging of ptpn6^-/- mutant 4dpf embryos in tg(mpx:GFP/mpeg:mCherry) background shows neutrophils in green and macrophages in red. Embryos were anesthesized with tricaine and tails were transsected distal to the notochord by scalpel blade. Embryos were mounted immediately for confocal imaging and imaged every minute from ∼30 min post amputation onwards for 7 h. Representative movie is shown.