Movie 1.

Movement of mKif6(1-493)-mNG and KIF1A (1-393)-LZ-HaloJFX554 along taxol-stabilized microtubules in vitro, shown in Fig. S3B. Cell lysates from COS-7 cells expressing mKif6(1-493)-mNG (green) or KIF1A (1-393)-LZ-HaloJFX554 (red) were added to a flow cell containing HiLy647-labeled taxol-stabilized microtubules (magenta). Images of KIF1A (1-393) were acquired at 1 frame every 100 ms for 61 frames and then images of Kif6(1-493) on the same microtubules were acquired at 1 frame every 3 s for 61 frames. The display rate of the movie is 30 fps.

Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells

Maki Takagishi, Yang Yue, Ryan S. Gray, Kristen J. Verhey, and John B. Wallingford

Disease Models and Mechanisms 2024. 17:None-None; doi: 10.1242/dmm.050137