Movie 1.

Movement of mKif6(1-493)-mNG and KIF1A (1-393)-LZ-HaloJFX554 along taxol-stabilized microtubules in vitro, shown in Fig. S3B. Cell lysates from COS-7 cells expressing mKif6(1-493)-mNG (green) or KIF1A (1-393)-LZ-HaloJFX554 (red) were added to a flow cell containing HiLy647-labeled taxol-stabilized microtubules (magenta). Images of KIF1A (1-393) were acquired at 1 frame every 100 ms for 61 frames and then images of Kif6(1-493) on the same microtubules were acquired at 1 frame every 3 s for 61 frames. The display rate of the movie is 30 fps.