Movie 8.

Live imaging of mCherry-expressing ependymal cell cilia at D14, related to Fig. 3J. Primary cultured ependymal cells form WT LV walls were cultured on glass-bottom dishes and transfected with mCherry at D12. At D14, time-lapse imaging of DIC was acquired at 190 fps for 2 sec. mCherry signal with DIC image is shown in the first flame.

Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells

Maki Takagishi, Yang Yue, Ryan S. Gray, Kristen J. Verhey, and John B. Wallingford

Disease Models and Mechanisms 2024. 17:None-None; doi: 10.1242/dmm.050137