Movie 2.

The beating heart of a Xenopus embryo injected with Co MO. Four-cell-stage embryos were injected in one dorsal blastomere with 7.5 ng Co MO in combination with 50 pg mGFP RNA as a lineage tracer. The embryos were anesthetized with 0.1x MBS (modified Barth's saline) containing 0.01% benzocaine. Beating hearts were recorded at stage 44. A representative embryo is shown from the ventral side. The embryo shows a dynamically beating heart; the blood flow through the ventricle and outflow tract is visible.

Fbrsl1 is required for heart development in Xenopus laevis and de novo variants in FBRSL1 can cause human heart defects

Hanna Berger, Sarah Gerstner, Marc-Frederik Horstmann, Silke Pauli, and Annette Borchers

Disease Models and Mechanisms 2024. 17:None-None; doi: 10.1242/dmm.050507