To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Play VideoPlayMuteCurrent Time 0:00/Duration Time 0:00Loaded: 0%Progress: 0%Stream TypeLIVERemaining Time -0:00 Playback Rate1ChaptersChaptersdescriptions off, selectedDescriptionssubtitles off, selectedSubtitlescaptions off, selectedCaptionsAudio TrackFullscreenThis is a modal window. Captions Settings Dialog Beginning of dialog window. Escape will cancel and close the window. Text Color White Black Red Green Blue Yellow Magenta Cyan Transparency Opaque Semi-Opaque Background Color Black White Red Green Blue Yellow Magenta Cyan Transparency Opaque Semi-Transparent Transparent Window Color Black White Red Green Blue Yellow Magenta Cyan Transparency Transparent Semi-Transparent Opaque Font Size 50% 75% 100% 125% 150% 175% 200% 300% 400% Text Edge Style None Raised Depressed Uniform Dropshadow Font Family Proportional Sans-Serif Monospace Sans-Serif Proportional Serif Monospace Serif Casual Script Small Caps Defaults Done Movie 3. A magnified view of 4T1 cells within a spheroid invading in 3D culture conditions. This video is a zoomed and cropped view of Movie 2 centered around the bottom edge of the spheroid. The play rate is 16 frames/sec (4,800 X real time). Arrows in the final frame indicate cells that correspond to the immunostained image in Fig. 7 of the manuscript. The highly metastatic 4T1 breast carcinoma model possesses features of a hybrid epithelial/mesenchymal phenotype Mary E. Herndon, Mitchell Ayers, Katherine N. Gibson-Corley, Michael K. Wendt, Lori L. Wallrath, Michael D. Henry, and Christopher S. Stipp Disease Models and Mechanisms 2024. 17:None-None; doi: 10.1242/dmm.050771 Download Original Video