Corresponding to Fig. 5. FLI-06 does not inhibit export of pre-accumulated cargo. Hela cells transfected with VSVG-EYFP and Sec24-mCherry were incubated at 40°C over night, then treated with GCA and nocodazole for 20-30 min on ice. Cells were shifted to 32°C and incubated for 45 min to induce gERES. After 45 min incubation at 32°C, GCA and nocodazole were washed out and incubated with 10 mM FLI-06 at 32°C. Images were taken every 5 sec on a Zeiss spinning disk confocal microscope. Arrows indicate VSVG-EYFP leaving Sec24-mCherry labelled ERES in the presence of FLI-06.