Movie 2

Corresponding to Fig. 5. FLI-06 does not inhibit export of pre-accumulated cargo. Hela cells transfected with VSVG-EYFP and Sec24-mCherry were incubated at 40°C over night, then treated with GCA and nocodazole for 20-30 min on ice. Cells were shifted to 32°C and incubated for 45 min to induce gERES. After 45 min incubation at 32°C, GCA and nocodazole were washed out and incubated with 10 mM FLI-06 at 32°C. Images were taken every 5 sec on a Zeiss spinning disk confocal microscope. Arrows indicate VSVG-EYFP leaving Sec24-mCherry labelled ERES in the presence of FLI-06.

Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06

Yoji Yonemura, Xiaolin Li, Katja Müller, Andreas Krämer, Paul Atigbire, Torben Mentrup, Talitha Feuerhake, Torsten Kroll, Olga Shomron, Richard Nohl, Hans-Dieter Arndt, Christian Hoischen, Peter Hemmerich, Koret Hirschberg, and Christoph Kaether

J Cell Sci 2016. 129:3868-3877; doi: 10.1242/jcs.186163