HeLa cells stably expressing Ig-mCherry (middle panels) were transiently transfected with expression plasmids encoding GFP-tagged ARHGEF10 (left panels). Merged images are shown in right panels. Boxed area indicate the image used in Figure 5D. These images were obtained using confocal microscopy. Time lapse images of these cells were taken at maximum speed. Scale bar, 5 μm.