Movie 1

Live visualization of CsA-treated CHO cells co-expressing Opn SP-RFP-PrP (transiently) that efficiently enters the ER and ΔSP-YFP-PrP (stably) stays entirely cytosolic. The movie shows that cytosolic PrP (yellow) molecules do not accumulate in existing aggresomes (red, arrow). Corresponding figure, 2A.