To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Supplementary Movie 1 3D model of PIN arises from single cells. Movie was taken 2 hours after RWPE-1 cells were seeded on the Matrigel for 64 hrs. Notice that instead of moving into each other, single cells were dividing and rotating to form the structure. A basal cell defect promotes budding of prostatic intraepithelial neoplasia Mengdie Wang, Raymond B. Nagle, Beatrice S. Knudsen, Gregory C. Rogers, and Anne E. Cress J Cell Sci 2017. 130:104-110; doi: 10.1242/jcs.188177 Download Original Video