To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Supplementary Movie 3 Spindle orientation of the basal cells in spheroid. Representative Z stack of a day 10 RWPE-1 spheroid immunostained with CEP135 (Red) as a centrosome marker and α-tubulin (green) with 0.2 μm step size. Scale bar = 15 μm. A basal cell defect promotes budding of prostatic intraepithelial neoplasia Mengdie Wang, Raymond B. Nagle, Beatrice S. Knudsen, Gregory C. Rogers, and Anne E. Cress J Cell Sci 2017. 130:104-110; doi: 10.1242/jcs.188177 Download Original Video