To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Supplementary Movie 4 Mature WT RWPE-1 spheroid formation without invasive phenotype. Movie was taken 12 days after RWPE-1 cells were seeded in 3D culture for 37 hrs. A basal cell defect promotes budding of prostatic intraepithelial neoplasia Mengdie Wang, Raymond B. Nagle, Beatrice S. Knudsen, Gregory C. Rogers, and Anne E. Cress J Cell Sci 2017. 130:104-110; doi: 10.1242/jcs.188177 Download Original Video