Supplemental Movie
Mitotic spindle orientation is disturbed in ZO-1 KD cells. Movie shows live imaging of cysts grown from WT (control) and ZO-1 KD (ZO-1 kd) cells expressing Lifeact-sfGFP (green) and H2B-mCherry (red). Arrows indicated mitotic events (movie pauses at these points).
ZO-1 interactions with F-actin and occludin direct epithelial polarization and single lumen specification in 3D culture
J Cell Sci 2017. 130:243-259; doi: 10.1242/jcs.188185