Supplemental Movie

Mitotic spindle orientation is disturbed in ZO-1 KD cells. Movie shows live imaging of cysts grown from WT (control) and ZO-1 KD (ZO-1 kd) cells expressing Lifeact-sfGFP (green) and H2B-mCherry (red). Arrows indicated mitotic events (movie pauses at these points).

ZO-1 interactions with F-actin and occludin direct epithelial polarization and single lumen specification in 3D culture

Matthew A. Odenwald, Wangsun Choi, Aaron Buckley, Nitesh Shashikanth, Nora E. Joseph, Yitang Wang, Michael H. Warren, Mary M. Buschmann, Roman Pavlyuk, Jeffrey Hildebrand, Ben Margolis, Alan S. Fanning, and Jerrold R. Turner

J Cell Sci 2017. 130:243-259; doi: 10.1242/jcs.188185