VEGF induces dynamin-independent internalisation of VEGFR2. (Related to Figure 1)
HUVECs transiently expressing VEGFR2-mCherry were monitored by TIRF microscopy. Dynasore and VEGF were added sequentially at the time points indicated in the movie. Penetration depth was set to 150 nm, images were acquired every 15 sec and reconstructed into a movie. Note that addition of dynasore (at t=00:06:29) leads to an increase of the signal of VEGFR2 at the plasma membrane (due to inhibition of constitutive internalisation), while the subsequent addition of VEGF (at t=00:54:24) results in progressive loss of the plasma membrane signal of VEGFR2 (due to VEGF-induced internalisation in a dynamin-independent manner).