VEGF induces membrane ruffling followed by internalisation of VEGFR2 into large vesicles. (Related to Figure 2)
HUVE cells transiently expressing GFP-actin and VEGFR2-mCherry were monitored by live cell time-lapse confocal microscopy in the presence of VEGF. Arrows indicate sites at the plasma membrane that undergo extensive ruffling (seen by the dynamics of GFP-actin) and give rise to enlarged VEGFR2-positive vesicles (seen by VEGFR2-mCherry). A magnified region of the plasma membrane is shown in the inset video at the top left corner of the movie. Plasma membrane ruffling starts 10 min following VEGF stimulation.