Movie S2

Mounting of a frustum on to a pin.
A small blob of conductive epoxy glue is first placed on the pin. After gently dislodging the frustum from the ParafilmTM (with the back of the frustum pointed upwards), a toothpick is used to lift it out of the ParafilmTM by placing a tip coated with conductive glue onto the back (i.e. opposite from the cell containing face). The frustrum is then inverted so the face points upward, and the back placed onto the blob of conductive glue on the pin. A small amount of the glue was then brought up around the block sides (not shown).

3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

Matthew R. G. Russell, Thomas R. Lerner, Jemima J. Burden, David O. Nkwe, Annegret Pelchen-Matthews, Marie-Charlotte Domart, Joanne Durgan, Anne Weston, Martin L. Jones, Christopher J. Peddie, Raffaella Carzaniga, Oliver Florey, Mark Marsh, Maximiliano G. Gutierrez, and Lucy M. Collinson

J Cell Sci 2017. 130:278-291; doi: 10.1242/jcs.188433