Movie 1

Example of a 3D reconstruction of a MDCK cyst stably expressing GFP-PRL-3 WT (green) grown for 72h and stained with Hoechst (blue), phalloidin (red) and ZO-1 antibody (magenta). Different points of view of a lateral asymmetric invasive ectopic lumen are shown, which has grown to the inside of one cell and is surrounded by ZO-1.