Video 4

Actin accumulation does not occur around midbodies in bec-1 mutants. Time-lapse movie showing that actin does not accumulate around the P0 midbody (arrowhead) in Class III PI3Kinase bec-1 mutants. Note that the midbodies are also not internalized. The strain expresses fluorescent reporters for non-muscle myosin (NMY-2::GFP) to label midbodies (yellow) and LifeAct::RFP to label F-actin (cyan). The right panel shows actin alone. Time-lapse data were collected during the 4-cell stage every 20 seconds for 12 minutes (5 fps). 3 Zs are projected (Z interval 1.2 μm).

C. elegans midbodies are released, phagocytosed and undergo LC3-dependent degradation independent of macroautophagy

Gholamreza Fazeli, Michaela Trinkwalder, Linda Irmisch, and Ann Marie Wehman

J Cell Sci 2016. 129:3721-3731; doi: 10.1242/jcs.190223