Movie S2

Activation of TRPML evokes global Ca²⁺ signals in fibroblasts.
Effect of 20 βM ML-SA1 on cytosolic Ca²⁺ levels of Fura-2 loaded fibroblasts. Images were acquired every 3 s and played back at a rate of 40 frames per second. Warmer colours represent an increase in Fura-2 fluorescence ratio which is proportional to Ca²⁺ concentration.