Confocal section through a cell expressing mRFP-RBD to label activated Ras (red), and GFP-talC63 (green) that accumulates in the direction of actin flow. The focus is placed 1.5 μm above the substrate surface. The same cell is shown in Figure 7A. Frame-to-frame interval 2.50 s. The 30.00-s image corresponds to the 0-s frame in Figure 7A.