Movie 3

Polarization of the B cell MTOC polarizes towards anti-Ig-coated beads. A20 cells that had been transfected with GFP-α-tubulin were mixed with 4.5-μm diameter anti-IgG-coated beads. Time-lapse confocal microscopy was used to acquire images every 2.5 s between 3 min and 8 min after adding the beads to the cells. The video is played back at 40 frames per second (100X real time).

The Rap1–cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse

Jia C. Wang, Jeff Y.-J. Lee, Sonja Christian, May Dang-Lawson, Caitlin Pritchard, Spencer A. Freeman, and Michael R. Gold

J Cell Sci 2017. 130:1094-1109; doi: 10.1242/jcs.191858