Movie 6

Cofilin inhibitory peptides prevent MTOC reorientation to the anti-Ig contact site. A20 cells expressing and GFP-α-tubulin (green) and F-tractin-tdTomato (red) were treated with the control Q peptide (5 μM) or the M/W cofilin-inhibitory peptides (5 μM each) and added to anti-IgG-coated coverslips. Time-lapse spinning disk confocal microscopy was used to acquire xy images of the contact site between the cell and the anti-IgGcoated coverslip every 10 s between 1 min and 8.5 min after adding the cells to the coverslips. The videos are played back at 10 frames per second (100X real time).

The Rap1–cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse

Jia C. Wang, Jeff Y.-J. Lee, Sonja Christian, May Dang-Lawson, Caitlin Pritchard, Spencer A. Freeman, and Michael R. Gold

J Cell Sci 2017. 130:1094-1109; doi: 10.1242/jcs.191858