Supplemental Movie 1

LIPUS stimulation induces actin-rich Circular Dorsal Ruffles.
Two-hour movie from live-cell imaging of a B16 GFP-actin cell, recorded in 60 s intervals. An ethanol-sterilised LIPUS transducer is placed in the media throughout the movie, allowing only fluorescence imaging. LIPUS is started at the end of the first hour, which rapidly leads to rearrangement of the actin cytoskeleton into a circular dorsal ruffle. Scale bar indicates 20 μm.

Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity

Paul Atherton, Franziska Lausecker, Andrew Harrison, and Christoph Ballestrem

J Cell Sci 2017. 130:2277-2291; doi: 10.1242/jcs.192781