Movie S1

Yeast cells exhibiting the BRCA1 aggregate are blocked in G1. NUP133-GFP cells expressing BRCA1-mCherry were pre-stimulated with galactose for 4 hours, spread on a slide covered with an agarose pad containing galactose and immobilized with a coverslip. Then, galactose stimulation was monitored by fluorescent videomicroscopy for 6 more hours. Movies were analyzed by spotting isolated cells at the beginning of the record, called “mother cells” and by measuring different parameters of these mother cells.