Cells expressing BRCA1 form a mix of non-dividing and slow dividing cells. Cells were pre-stimulated with galactose for 4 hours, spread on a slide covered with an agarose pad containing galactose and immobilized with a coverslip. Vector cells were used as a control. Then, galactose (GAL) stimulation was monitored using transillumination videomicroscopy for 9 more hours. Movies were analyzed as in Movie S1.
Pierre Thouvenot, Lou Fourrière, Elodie Dardillac, Barbara Ben Yamin, Aurianne Lescure, Vincent Lejour, Xavier Heiligenstein, Jean-Baptiste Boulé, Maryse Romao, Graça Raposo-Benedetti, Bernard S. Lopez, Alain Nicolas, and Gaël A. Millot
J Cell Sci
2016. 129:4366-4378; doi: 10.1242/jcs.192880