Movie S3

Time-lapse acquisition of HeLa cells expressing Flag-CCR2B (white) and FLNa-GFP (green) and Rab4-Cherry (red) acquired as in Figure 3. HeLa cells were transfected with the corresponding plasmids, pre-labeled with anti-Flag followed by Alexa647 at anti-mouse antibody 4°C and immediately followed by live confocal imaging after 20 nM CCL2. Representative vesicles are shown from a single cell.