Movie S4

Time-lapse acquisition of HeLa cells expressing Flag-CCR2B (white) and FLNa-GFP (green) and Rab5-Cherry (red) acquired as in Figure 3. HeLa cells were transfected with the corresponding plasmids, pre-labeled with anti-Flag followed by Alexa647 at anti-mouse antibody 4°C and immediately followed by live confocal imaging after 20 nM CCL2. Representative vesicles are shown from a single cell.

Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling

Mònica Pons, Ismael Izquierdo, Mireia Andreu-Carbó, Georgina Garrido, Jesús Planagumà, Olivia Muriel, Miguel A. del Pozo, M. Isabel Geli, and Anna M. Aragay

J Cell Sci 2017. 130:490-501; doi: 10.1242/jcs.193821