Aggregation of HSB1HectPH1- cells plated on agar. Starving HSB1HectPH1- cells were plated on Soerensen phosphate agar at a concentration of 6.4 x 105 per cm2 and incubated at 22° C. After 7 hours incubation, a time lapse movie was recorded for 50 min, using Lumenera Infinity 3 camera mounted to a Zeiss Axiovert 200 microscope, with a 10x objective, with photograms taken at intervals of 15 sec.