Supplemental Movie 3

3D volume view of meiosis I cell. A 3D volume view of a metaphase I cell rotates around the y-axis to show the depth of the z-dimension and demonstrate that cells were not compressed between the coverslip and slide. The cell volume is illuminated with SYTO12 (green) and the metaphase I chromosomes (bright green) can be seen aligned in the middle of the cell volume. All movies captured a 20μm z-dimensional cross-section of meiotic cells. The grid scale is 10 μm.