Movie SM1

Motility of Cin8 on immobilized, polarity-marked GMPCPP-stabilized MTs.
Cin8 undergoes fast minus-end–directed motility and clusters at the minus-end of the MTs. Clusters of Cin8 undergo slow plus-end–directed motility. Left: immobilized GMPCPP-stabilized red MTs, yellow labels the MT plus-end; Right: Cin8. Bar: 10 μm. Time interval between frames: 1.28 s. Movie sped up 12.8X, 10 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040