Motility of Cin8 on immobilized, polarity-marked GMPCPP-stabilized MTs.
Cin8 undergoes fast minus-end–directed motility and clusters at the minus-end of the MTs. Clusters of Cin8 undergo slow plus-end–directed motility. Left: immobilized GMPCPP-stabilized red MTs, yellow labels the MT plus-end; Right: Cin8. Bar: 10 μm. Time interval between frames: 1.28 s. Movie sped up 12.8X, 10 fps.