Movie SM2

Cin8 tracks the minus-end of dynamic MTs.
Cin8 tracks the MT minus-end (MT#1). MT#2: no Cin8 tracking of the MT ends. Yellow: GMPCPP-stabilized seeds; Red: dynamic MTs; White: Cin8. Plus- and minus-ends of MTs are indicated, based on the difference in dynamics at the two ends (the plus-end of MT#1 is free in solution and only visible in some frames). Bar: 10 μm. Time intervals between frames: 3.3 s. Movie sped up 30X, 9 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040