Movie SM4

Plus-end–directed Cin8 cluster, moving on a dynamic MT, splits into several clusters, some of which are moving in the minus-end direction of the MT.
Movie relates to Fig. 2E, upper left panel. Only Cin8 (GFP channel) is shown, the directionality of the MT is indicated based on the different dynamics of the plus- and minus-ends. Arrowheads mark the location of clusters; change in the arrowhead color indicates a cluster-splitting event. Bar: 3 μm. Time intervals between frames: 3.4 s. Movie sped up 34X, 10 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040