Movie SM5

Cin8 cluster-mediated antiparallel MT capturing and subsequent sliding.
Red MT with yellow label of the plus-end is immobilized to the glass surface. Yellow MTs with red label of the plus-end are free in solution. The different channels are indicated. Cin8 cluster that is bound to the minus-end of the red MT mediates the capturing and subsequent antiparallel sliding of the yellow MT. Bar: 2 μm. Time interval between frames: 3.7 s. Movie sped up 30X, 8 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040