Movie SM6

Cin8-mediated antiparallel MT sliding.
Red MTs are immobilized to the glass surface, yellow MTs are free in solution; Cin8 is shown in white. Left panel: immobilized and free MTs; right panel: immobilized MTs and Cin8. MTs#1-3: Cin8 is concentrated at the leading minus-end of the sliding MTs. MT#1: two antiparallel MTs are initially attached by Cin8 at their minus-end; MT#3: a sliding MT is switching between two nearly perpendicular red MTs. This switch is mediated by the Cin8 cluster located at the minus-end of the sliding yellow MT. Bar: 10 μm. Time interval between frames: 3.6 s. Movie sped up 40X, 11 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040