MT capturing and pivoting during antiparallel sliding, mediated by a cluster located at the minus-end of the immobilized (red) MT.
Red MTs are immobilized to the glass surface, yellow MTs are free in solution; Cin8 is shown in white. Left panel: immobilized and free MTs and Cin8; right panel: immobilized MTs and Cin8. The yellow MT undergoes antiparallel sliding, mediated by a cluster of Cin8 located at the minus-end of the red MT. The yellow MT pivots around the minus-end located Cin8 cluster. Minus- and plus end of the red MT are indicated based on the location of the Cin8 cluster. Bar: 5 μm. Time interval between frames: 3.6 s. Movie sped up 36X, 10 fps.